Study on the diagnostic value and efficacy of hypersensitive C-reactive protein combined with SSA in different SARS-CoV-2 variants
Objective To explore the auxiliary diagnostic value and efficacy of hypersensitive C-reactive protein(hs-CRP)combined with serum amyloid A(SAA)in different SARS-CoV-2 variants,so as to provide reference for clinical diagnosis and treatment.Methods A total of 323 COVID-19 patients from February 2022 to April 2023 were selected as the observation group.Fluorescence quantitative RT-PCR and third generation nanopore high-throughput whole genome sequencing technology were used to complete the identification of SARS-CoV-2 variants.91 persons took regular physical examination were selected as the control group.The level of hs-CRP and SAA were measured by using immunoturbidimetry and latex turbidimetry.The ROC curve was drawn to analyze the auxiliary differential diagnostic efficacy of hs-CRP and SAA in different SARS-CoV-2 variants.Results The levels of hs-CRP and SAA of COVID-19 patients in the observation group were(14.51±3.21)mg/L and(19.98±3.25)mg/L,were higher than those in the control group(P＜0.05).The levels of hs-CRP and SAA of COVID-19 patients with different genders had no statistical difference(P＞0.05).The levels of hs-CRP and SAA in patients aged ≥60 years old were higher than those in patients aged＜60 years(P＜0.05).The top two variant types were BA.1 and BF.7.14,with 63 and 56 cases respectively.All the confirmed COVID-19 cases completed the identification of virus variants,the levels of hs-CRP and SAA of different variants were statistically different(P＜0.05).Among them,the patients infected BF.7.14.4 variant had the higher levels of hs-CRP and SA A,followed by BA.5.1.3 variant,while the patients infected BF.7.14 variant had the lower levels of hs-CRP[(4.52±0.61)mg/L]and SAA[(8.54±0.81)mg/L].ROC curve showed that AUC value was 0.875,the sensitivity and specificity of hs-CRP combined with SAA were higher than that of single hs-CRP(AUC value=0.735)and SAA(AUC value=0.792)in different SARS-CoV-2 variants(P＜0.05).Conclusion The levels of hs-CRP and SAA were higher than normal in COVID-19 patients,and there were differences in the levels of different variants infection.The combined determination of hs-CRP and SAA could improve the early diagnosis of COVID-19 and the identification efficiency of different SARS-CoV-2 variants.
Hypersensitive C-reactive proteinSerum amyloid protein ACOVID-19SARS-CoV-2Variant
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