Study on antibiotic resistance characteristics and mecA gene detection of food-borne Staphylococcus aureus in Ningxia
Objective To detect the mecA gene and analyze the drug resistance of Staphylococcus aureus isolated from food safety risk surveillance in Ningxia,to understand the drug resistance trend and mecA distribution characteristics in Ningxia over the past 10 years,to provide theoretical basis and technical reference for effective prevention and control of the spread and infection of the bacterium.Methods A total of 195 S.aureus strains isolated from food safety risk surveillance in Ningxia from 2011 to 2021 were collected.The nuc and mecA genes were detected simultaneously by using multiplex fluorescent PCR method.The drug susceptibility of 15 antibiotics was tested by the broth dilution method.Results Among 195 strains of S.aureus,19 strains carried mecA gene,the positive rate was 9.74％.It was completely resistant to penicillin with a resistance rate of 100.00％,followed by erythromycin and clindamycin with resistance rates of 62.56％and 46.67％.The most sensitive drugs were teicolanin and rifampicin,with sensitivity rates of 100.00％,followed by daptomycin,linezolid,vancomycin,and cotrimoxazole.A total of 195 strains of S.aureus formed 40 drug resistance profiles,and 146 strains were multi-drug resistant.Conclusion From 2011 to 2021,mecA gene was positive in food-borne S.aureus in Ningxia.Drug resistance should continue to be strengthened,at the same time,attention should be paid to vancomycin resistance,effective prevention and control measures should be taken actively.
Food-borne Staphylococcus aureusMecA geneCharacteristics of drug resistance
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