Study on the bioaerosol sampling methods and the distribution of microorganisms in hospital air
Objective To understand the temporal and spatial distribution of microorganisms such as Staphylococcus aureus and influenza virus in the air of hospital environment,in order to provide support for hospital infection research,risk analysis and sterilization and disposal.Methods From February to April 2023,air samples were collected from four locations,including the neonatal ICU,neonatal internal medicine ward,the lobby on the second floor of the outpatient building,and respiratory ward of a hospital in Beijing,using six-stage Anderson microbiological impactor and cyclonic bioaerosol sampler.The collected frequency was twice a week,and a total of 292 samples were collected,of which 146 were used for bacterial culture and another 146 were used for virus enrichment.Multiple Real-time fluorescence quantitative PCR method was used to screen 6 kinds of bacteria such as Staphylococcus aureus and 12 kinds of viruse such as influenza virus.Results Out of 146 bacterial culture samples,30 were tested positive for Staphylococcus aureus(20.55％),16 were positive for Pseudomonas aeruginosa(10.96％),and 2 were positive for Klebsiella pneumoniae(1.73％).Out of 146 virus enriched samples,1(0.68％)was positive for influenza A virus and 1(0.68％)was positive for influenza B virus.The positive rate of Staphylococcus aureus was the highest in March(24.07％),while the positive rate of Pseudomonas aeruginosa was highest in February(14.71％).The positive rate of Staphylococcus aureus was the highest in the lobby on the second floor of the outpatient building(22.73％),the positive rate of Pseudomonas aeruginosa was the highest in the neonatal ICU(28.57％).Among them,there was a statistically significant difference in the positive rate of Staphylococcus aureus in different sampling months(P=0.027).Conclusion From February to April 2023,the highest positive rate of Staphylococcus aureus related to hospital infection was found in the air environment of a hospital in Beijing,followed by Pseudomonas aeruginosa and Klebsiella pneumoniae.
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