采用DNA条形码技术鉴定口岸输入性鼠形动物
Application of DNA barcoding technology for identification of imported murine animal at ports
孙玉婷 1田锋 1易海华 2杨国平 2张荣 2田玲玲 2孟媛 2杨庆贵 2耿合员2
作者信息
- 1. 新疆国际旅行卫生保健中心(乌鲁木齐海关口岸门诊部),新疆乌鲁木齐 830011
- 2. 江苏国际旅行卫生保健中心(南京海关口岸门诊部)
- 折叠
摘要
目的 针对形态上难以识别的境外输入性鼠形动物,探索DNA条形码技术鉴定鼠种的可行性.方法 江苏口岸收集1份输入性且在形态上难以鉴别的鼠形动物,通过PCR法扩增线粒体细胞色素C氧化酶Ⅰ基因(COⅠ),目的片段经测序后进行序列比对分析,计算物种种间和种内的遗传距离,采用邻近法构建系统发育树.结果 序列比对显示该输入性鼠形动物为黑家鼠(Rattus rattus);系统发育分析显示,物种种内遗传距离为7.7％,种间距离为11.6％～21.0％,该输入性鼠形动物在进化树上形成独立的进化分支.结论 DNA条形码技术能准确鉴定输入性鼠形动物,尤其是有助于提高在形态上难以鉴别的鼠形动物鉴定的准确性.
Abstract
Objective To explore the feasibility of DNA barcoding technique for identification of rat species,which is difficult to identify morphologically.Methods One murine animals imported from Jiangsu ports that was morphologically difficult to identify was collected.Mitochondrial cytochrome C oxidase Ⅰ(COⅠ)gene was amplified by PCR method,the target fragment was sequenced for sequence comparison analysis,and the genetic distance between species and within species was calculated.Results The sequence comparison showed that the imported rodent was Rattus rattus.Phylogenetic analysis showed that the intraspecific genetic distance was 7.7％,and the interspecific genetic distance was 11.6％～21.0％.The imported murine animal formed one independent evolutionary branch on the evolutionary tree.Conclusion DNA barcoding technology could accurately and effectively identify imported murine animals,especially help to improve the accuracy of identification of morphologically difficult murine animals.
关键词
DNA条形码/输入性/鼠形动物/鉴定Key words
DNA barcoding/Imported/Murine animal/Identification引用本文复制引用
基金项目
国家重点研发计划项目(2021YFC2301200)
新疆维吾尔自治区自然科学基金项目(2023D01A87)
海关总署科研项目(2023HK022)
南京海关科研项目(2023KJ02)
出版年
2024
NETL
NSTL
万方数据