济南市1例输入性奥密克戎毒株刺突糖蛋白结构和功能的生物信息学分析
Bioinformatics analysis of the structure and function of spike glycoprotein in an imported Omicron variant in Jinan city
刘保华 1白飒 1闫荣军 1赵辉 1滕新栋 2黄伟 3韩焕美1
作者信息
- 1. 济南国际旅行卫生保健中心(济南海关口岸门诊部),山东济南 250014
- 2. 青岛国际旅行卫生保健中心(青岛海关口岸门诊部)
- 3. 济南机场海关
- 折叠
摘要
目的 分析新冠病毒奥密克戎变异株刺突糖蛋白的结构和功能,为疫苗研发提供数据.方法 对济南市1例输入性新冠病毒奥密克戎毒株,采用二代测序技术进行全基因组测序,分析刺突糖蛋白基因序列;用生物信息学方法分析刺突糖蛋白氨基酸组成、理化性质、跨膜结构、磷酸化修饰、糖基化修饰、结构域和B细胞抗原表位等生物学特性.结果 测序获得1株输入性新冠病毒全基因组序列,基因组全长29 847 nt,毒株基因型为XBB.1.16.1;其刺突糖蛋白由1 269个氨基酸组成,存在1个跨膜螺旋区,主要分布于宿主细胞的内质网和细胞质膜中;有134个磷酸化位点、23个糖基化位点,3个蛋白结构域和11个可能的B细胞抗原表位,蛋白二级结构主要由无规则卷曲组成.结论 采用生物信息学方法分析奥密克戎变异株刺突糖蛋白结构与功能,为了解刺突糖蛋白生物学信息提供数据.
Abstract
Objective To analyze the structure and function of the spike glycoprotein of an SARS-CoV-2 Omicron variant,to provide data for vaccine development.Methods The whole genome was sequenced by the next-generation sequencing of 1 case imported Omicron variant in Jinan city,the sequence of spike glycoprotein gene was analyzed.The amino acid composition,physicochemical properties,transmembrane structure,phosphorylation modification,glycosy-lation modification,domain and B cell epitope were analyzed by the bioinformatics methods.Results The whole genome sequence with 29 847 nt was obtained,the strain was Omicron XBB.1.16.1 variant.The spike glycoprotein was composed of 1 269 amino acids,with one transmembrane helix region,was distributed mainly in the endoplasmic reticulum membrance and plasma membrane of host cell.This protein had 134 potential phosphorylation sites,23 pos-sible glycosylation sites,3 conserved domains and 11 copies of B cell antigen epitope.The secondary structure of the spike glycoprotein was mainly consisted random coils.Conclusion The structure and function of the spike glycopro-tein of Omicron variant were analyzed by the bioinformatics methods,which provided an important data for under-standing the biological functions of the spike glycoprotein.
关键词
奥密克戎变异株/刺突糖蛋白/生信息学分析Key words
Omicron variant/Spike glycoprotein/Bioinformatics analysis引用本文复制引用
基金项目
山东省科技厅项目(2021CXGC011306)
海关总署科研项目(2021HK139)
济南海关科研项目(2020JK003)
出版年
2024
NETL
NSTL
万方数据