Objective To analyze the structure and function of the spike glycoprotein of an SARS-CoV-2 Omicron variant,to provide data for vaccine development.Methods The whole genome was sequenced by the next-generation sequencing of 1 case imported Omicron variant in Jinan city,the sequence of spike glycoprotein gene was analyzed.The amino acid composition,physicochemical properties,transmembrane structure,phosphorylation modification,glycosy-lation modification,domain and B cell epitope were analyzed by the bioinformatics methods.Results The whole genome sequence with 29 847 nt was obtained,the strain was Omicron XBB.1.16.1 variant.The spike glycoprotein was composed of 1 269 amino acids,with one transmembrane helix region,was distributed mainly in the endoplasmic reticulum membrance and plasma membrane of host cell.This protein had 134 potential phosphorylation sites,23 pos-sible glycosylation sites,3 conserved domains and 11 copies of B cell antigen epitope.The secondary structure of the spike glycoprotein was mainly consisted random coils.Conclusion The structure and function of the spike glycopro-tein of Omicron variant were analyzed by the bioinformatics methods,which provided an important data for under-standing the biological functions of the spike glycoprotein.
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