珠海口岸首次检出的输入性猴痘病毒全基因组序列特征分析
Genomic characterization of the first detected imported monkeypox virus at Zhuhai port
黄慧娜 1周海棠 1欧姣 1袁成 1陈新彬 1涂承宁 1汪海波1
作者信息
- 1. 珠海国际旅行卫生保健中心(拱北海关口岸门诊部),广东珠海 519020
- 折叠
摘要
目的 了解珠海口岸首例输入性猴痘病毒基因组特征和遗传变异情况,为口岸猴痘防控工作提供技术支撑.方法 提取珠海口岸首次检出的输入性猴痘病例咽拭子样本核酸,采用猴痘全基因组测序建库辅助试剂盒进行多重PCR靶向扩增病毒全基因组序列,并利用纳米孔三代测序仪开展测序,使用生物信息学软件对毒株的全基因组序列特征、毒株分型和变异位点进行分析.结果 珠海口岸首例输入性猴痘病毒序列全长197 177 nt,与参考序列(NC_063383.1)核苷酸同源性为95.7％,含有36个氨基酸突变,涉及宿主免疫调控、病毒表面结构等多种功能改变.系统进化分析显示,与LC760460(日本,2023)、OR855744(韩国,2023)、LC753968(日本,2022)聚类在同一分支,均属Ⅱb C.1谱系.结论 珠海口岸首次检出的输入性猴痘病毒属Ⅱb C.1谱系,共有36个氨基酸突变,应持续开展猴痘病毒及其基因变异监测.
Abstract
Objective To investigate the genomic characteristics and genetic variation of the first imported monkeypox virus(MPXV)at Zhuhai port,with the aim to provide technical support for the prevention and control of Mpox at ports.Methods The nucleic acid was extracted from oral swab sample of the first imported MPXV infected arriving passenger at Zhuhai port.The whole genome of the virus was amplified using multiplex PCR method provided by the MPXV NGS library construction auxiliary kit and sequenced by nanopore third-generation sequencer.The bioinformatics softwares were applied to analyze the genetic characteristics,subtype and mutations of the genome.Results The sequence length of the first imported MPXV at Zhuhai port was 197 177 nt,and the nucleotide homology with the reference sequence(NC_063383.1)was 95.7％.Compared with the reference genome(NC_063383.1),there were 36 amino acid mutations,involving multiple functional changes such as host immune regulation and virus surface structure.Phylogenetic analysis showed that the virus clustered with LC760460(Japan,2023),OR855744(South Korea,2023)and LC753968(Japan,2022)tightly,all of which belonged to the Ⅱb C.1 lineage.Conclusion The MPXV detected for the first time at Zhuhai port belonged to Ⅱb C.1 lineage and contained 36 amino acid mutations,calling for the continuous surveillance of MPXV and its genomic variation.
关键词
猴痘病毒/全基因组序列/进化分析Key words
Monkeypox virus/Genomic sequence/Phylogenetic analysis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据