目的 对乌鲁木齐市2022年3月一起诺如病毒暴发疫情进行病原学鉴定，基于全基因组序列进行基因分型及分子进化分析。方法 采用实时荧光RT-PCR法对送检的11份粪便样本进行诺如病毒核酸检测，采用二代测序技术对检测Ct值小于30的2份样本进行全基因组测序，用Mega 6。06软件对序列进行系统进化分析及主要衣壳蛋白1(major capsid protein 1，VP1)区氨基酸序列变异分析。结果 11份样本中有7份经实时荧光RT-PCR检测为诺如病毒GⅡ型阳性，2份样本全基因组序列经序列比对，为诺如病毒GⅡ。P7-GⅡ。6型，与2012年阿根廷株MW305613序列一致性最高，为95。79％，VP1区基因属于GⅡ。6a亚型;VP1区氨基酸序列与2015年中国株KU870455相比有4个氨基酸位点改变。结论 本次疫情样本中检出诺如病毒GⅡ。P7-GⅡ。6型，应持续开展对该型别诺如病毒的监测和分子特征研究。
Pathogen identification and characteristics of whole genome sequence analysis of a norovirus epidemic
Objective To identify the pathogen of a norovirus epidemic in March 2022 in Urumqi,to analyze the genotype and molecular characteristics by whole genome sequencing.Methods Real-time RT-PCR was used to screen pathogens in 11 fecal samples collected from the epidemic.Two samples with Ct value less than 30 were se-quenced by using next generation sequencing technology for whole genome sequencing.The Mega 6.06 software was used to analyze the phylogenetic evolution and amino acid sequence variation in the major capsid protein 1(VP1)region.Results Among 11 samples,7 samples were identified norovirus GⅡ positive.Two samples were sequenced and identified as norovirus GⅡ.P7-GⅡ.6 through sequence alignment,the sequence was shared the highest homology with the strain MW305613 reported in Argentina in 2012,and VP1 gene in this study belonged to GⅡ.6a group.There were 4 amino acid changes in VP1 region compared to the Chinese strain KU870455 reported in 2015.Con-clusion Norovirus GⅡ.P7-GⅡ.6 was detected positive in this epidemic continuous monitoring and molecular characteri-zation research of this type of norovirus should be carried out.
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