Armored RNA technology based on bacteriophage MS2 and its application in viral nucleic acid detection
The detection of viral RNA requires positive control or quantifiable standard.Initially,inactivated or attenu-ated pathogens and transcribed RNA in vitro were used for nucleic acid detection as positive controls.However,the use of pathogens poses biosafety risks,and RNA is prone to degradation and cannot be monitored throughout the en-tire process from nucleic acid extraction to detection.Therefore,a new technology,the"armored RNA technology",has been used for the detection of highly infectious and pathogenic RNA viruses.Bacteriophage MS2 is a single-stranded RNA virus that widely exists in nature.Armored RNA technology refers to the use of the capsid protein of bacterio-phage MS2 to encapsulate and protect the target RNA to improve the stability of the RNA.In this paper,the phage MS2-based armored RNA technology and its application in viral nucleic acid detection are reviewed.
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