Characterization of viral encephalitis and analysis of associated enterovirus genes in Hebei Province,2019-2021
Objective To exploring the epidemiological characteristics of viral encephalitis and related enterovirus gene characteristics in Hebei province.Methods The serum or cerebrospinal fluid samples from acute viral en-cephalitis cases in Hebei were collected from 2019 to 2021.ELISA and Real-time PCR were used to detect com-mon pathogens,virus isolation and VP1 region sequencing were performed in enterovirus positive samples.Results A total of 692 cases were collected in 2019-2021,with male to female ratio of 1.39∶1.97.54％of the cases was younger than 15 years old.There were statistically significant differences in incidence rates between different age groups(x2=2 719.663,P＜0.001).The cases were distributed throughout the year,with a relatively higher number of cases from May to July,accounted for 34.68％.The positive rate was 40.17％with 278 enterovirus positive cases.The most common pathogens were herpes simplex virus(37.71％),enterovirus(16.50％)and mumps virus(15.15％).Sequence analysis showed that the associated enteroviruses were mainly Echovirus 33(Echo33),Echo30,Echo9 and Cox-sackievirus B5(CVB5),which were closely related to the isolates in recent years in China.Conclusion Viral en-cephalitis in Hebei was characterized by certain population,seasonal characteristics and pathogen diversity.There were several genotypes of viral encephalitis associated enteroviruses,and no obvious variations had been found.
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