Association of dnmt3b gene polymorphism with HBV susceptibility and persistent infection in children
Objective To explore the relationship between DNA methyltransferase 3B(dnmt3b)gene polymorphism and the susceptibility and persistent infection of hepatitis B virus(HBV)in children,to provide novel basis and strategy for the prevention and treatment of hepatitis B.Methods A total of 104 children with HBV infection admitted to CUHK Huiya Hospital from June 2020 to February 2023 were selected,and 104 healthy children who underwent physical examination in the same hospital during the same period were selected as healthy control.PCR and DNA sequencing were used to detect the polymorphism of dnmt3b gene.According to the outcome within 6 months after treatment,the children were divided into persistent infection group and negative conversion group,the risk factors for persistent HBV infection were analyzed.Results Among 104 children with HBV infection,HBsAg turned negative in 78 children and remained positive in 26 children after anti-HBV treatment.The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and serum HBV DNA load in persistent infection group were(76.51±19.35)U/L,(72.34±20.06)U/L,(124.61±11.07)x103/ml were higher than those in the negative conversion group(P＜0.05).The frequency of GG gene in dnmt3b rs1550117 in the persistent infection group(73.08％)was higher than that in the negative conversion group(39.74％)(P＜0.05).ALT,AST,HBV DNA load,and dnmt3b rs1550117 GG genotype were the risk factors for persistent HBV infection in children with hepatitis B(P＜0.05).The frequency of GG gene in dnmt3b rs1550117 in the HBV infection group(40.08％)was higher than that in the healthy control group(20.19％)(P＜0.05).Conclusion Dnmt3b rs1550117 gene was associated with susceptibility and persistent infection in HBV infection children,and GG genotype HBV infection children had a higher risk of susceptibility.
Hepatitis B virusChildSusceptibilityPersistent infectionDnmt3bGenetic polymorphism
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NSTL
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