Research progress of host nucleic acid depletion methods for metagenomic sequencing
Metagenomic sequencing relying on the second-generation and third-generation high-throughput sequencing technologies has been widely employed in laboratory medicine,environmental science,ecology and microbiology.It has especially played a vital role in pathogen detection for infectious diseases.However,there is a limitation that a high host background will lead to reduced detection sensitivity,especially in samples like tissues,blood,swabs,silva,bile acid and purulent fluids.Insufficient sequencing depths derived from such samples might result in missing detection of causal pathogens or low-abundance microbes,and further a waste of sequencing resource.Therefore,in order to increase the ratio of microbial sequences and detection sensitivity,it's necessary to deplete host cells or nucleic acid before or after nucleic acid extraction.This review summarized the current host nucleic acid depletion methods and their advantages and disadvantages.
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