Evaluation of nanomagnetic beads with different modification on the rapid enrichment of pathogenic bacteria
Objective In order to meet the requirements of rapid detection at ports,nano magnetic beads with good adsorption effect on all pathogenic bacteria were screened,and the rapid adsorption performance of nano magnetic beads with different modifications was tested.Methods The rapid adsorption capacity of 11 chemically modified nano-magnetic beads for Escherichia coli and Staphylococcus aureus was determined,the magnetic beads with good adsorption function were obtained.The homogeneity,particle size and modification way of the nano magnetic were determined by scanning electron microscopy,transimission electron microscopy and Fourier transform infrared spectroscopy.Results The enrichment efficiency of Dyna magnetic beads for Escherichia coli and Staphylococcus aureus was(78.01±0.57)％and(71.98±1.19)％,respectively,and that of Si-PSA magnetic beads was(57.28±0.66)％and(89.10±2.40)％,respectively.The particle size of Dyna magnetic beads was about 500 nm,and the particle size of Si-PSA magnetic beads was about 200 nm.The particle size uniformity of both nano magnetic beads was good.Conclusion The nano magnetic beads with good adsorption effect on both pathogens were screened and could be used for rapid enrichment of pathogenic bacteria.
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