Identification of mosquito species collected at ports in high altitude by using DNA barcoding sequencing technology
Objective To identify the mosquitoes at high altitude ports by using DNA barcoding technology,and to provide scientific basis for mosquito classification and prevention and control of arboviral diseases.Methods From July to September 2023,mosquitoes were caught by UV light and double-layer tents at the lawn,sewage treatment plant and dormitory of Caojiapu Airport in Xining and Golmud Airport.The mosquitoes were identified by morpho-logical identification and DNA barcoding sequencing identification.Results A total of 110 mosquitoes were cap-tured,including 76 collected at Caojiapu Airport and 34 collected at Golmud Airport.All the species were identified as Culex pipiens pallens by morphological identification.Fifteen mosquitoes were randomly selected and DNA bar-coding molecular sequencing was performed.Sequencing blast showed that 14 were identified as Culex pipiens and 1 was Culex quinquefasciatus.Conclusion Using DNA barcoding sequencing molecular identification method,the pre-liminary exploration of mosquito species composition at ports in high-altitude was understood,provided more accurate scientific basis for medical vectors prevention and control at ports.
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