Study on the diagnostic value of Linc02555 expression in peripheral blood mononuclear cell for active pulmonary tuberculosis
Objective To investigate the diagnostic value of long intergenic non-coding RNA 02555(Linc02555)in the active pulmonary tuberculosis(TB)by detecting the expression level of Linc02555 in peripheral blood mononu-clear cells(PBMC)of TB and healthy controls(HC).Methods Transcriptome sequencing was performed on PBMC samples from 62 patients with active pulmonary TB and 46 healthy individuals.Based on the criteria of fold change≥ 1.5,Q-value ≤ 0.05,and average expression abundance(RPKM)≥ 1,The transcriptome level of Linc02555 in PBMCs of tuberculosis patients was significantly upregulated compared to healthy individuals.Subsequently,qPCR was used to validate the differential expression of Linc02555 in PBMCs from 24 healthy individuals,24 active tuberculo-sis patients and 21 pneumonia patients.Additionally,the expression levels of Linc02555 in PBMCs from 37 newly diagnosed tuberculosis patients,26 patients who received standard anti-TB treatment for 1 month,and 18 patients treated for 3 months were examined.Data processing and receiver operating characteristic(ROC)curve plotting were conducted using Graphpad Prism 8.0 to evaluate the diagnostic value of Linc02555 expression levels in active pul-monary TB,as assessed by the area under the curve(AUC).Results The transcriptome sequencing showed that the expression level of Linc02555(0.272±0.036)in PBMCs of patients with active pulmonary TB was significantly high-er than that in the healthy control group(0.103±0.011)(t=3.997,P＜0.001).qPCR validation further demonstrated that the expression level of Linc02555 in PBMCs of patients with active TB(1.639±0.280)was significantly higher than that of healthy controls(0.338±0.052)and pneumonia group(0236±0.105)(F=18.93,P＜0.000 1).Compared to the expression level of Linc02555 before anti-TB treatment(2.178±0.458),its expression level was significantly reduced after 1 month(0.198±0.087)and 3 months(0.242±0.120)of anti-TB treatment.ROC curve analysis showed that the AUC value of Linc02555 in dis-criminating active pulmonary TB from healthy controls was 0.86,with a sensitivity and specificity of 72.27％and 90.48％,respectively.The AUC value of Linc02555 in discriminating active pulmonary TB from pneumonia was 0.91,with a sensi-tivity and specificity of 90.91％and 85.00％,respectively.Conclusion Linc02555 is emerging as a promising potential molecular biomarker for the diagnosis and therapeutic monitoring of pulmonary TB.
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