首页|十种清热解毒中药提取物抗泛耐药鲍曼不动杆菌感染的研究

十种清热解毒中药提取物抗泛耐药鲍曼不动杆菌感染的研究

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目的 研究金果榄、矮脚苦蒿和姜黄等十种清热解毒中药抗泛耐药鲍曼不动杆菌(XDR-AB)感染的活性，筛选出具有体内抗感染活性的中药提取物，为发现新型抗XDR-AB感染的抗菌药物提供研究基础。方法 分别采用水、50％乙醇、95％乙醇提取十种清热解毒中药并制成不同浓度的药物浸膏，与课题组前期优化的XDR-AB感染秀丽隐杆线虫模型共孵育，通过线虫的存活率判断中药提取物的体内抗XDR-AB活性。结果 随着姜黄和土荆皮提取物浓度的增大，XDR-AB感染的线虫存活率不断提高。姜黄的水提取物、50％乙醇提取物和95％乙醇提取物在浓度1 000 μg/mL时，可使XDR-AB感染的秀丽隐杆线虫存活率分别提高至54。2％(与阴性对照组比较，P＜0。001)、18。8％、13。3％;土荆皮的水提取物、50％乙醇提取物和95％乙醇提取物在浓度1 000 μg/mL时，可使XDR-AB感染的秀丽隐杆线虫存活率分别提高至47。4％(与阴性对照组比较，P＜0。001)、23。8％、15。8％。结论 姜黄和土荆皮水提取物具有较好的抗XDR-AB感染活性，可对其主要化学成分进行体外抗菌药效验证，以期发现新型抗XDR-AB感染的抗菌药物。
Ten kinds of antipyretic-antidotal traditional Chinese medicine extracts against extensively drug-resistant Acinetobacter baumannii infection
Objective To study the activity of ten kinds of antipyretic-antidotal traditional Chinese medicine(TCM),including radix tinosporae.herb of blin conyza and turmeric,against extensively drug-resistant Acineto-bacter baumannii(XDR-AB)infection,screen out the extracts of antipyretic-antidotal TCM which have in vivo anti-infection activity,provide a research basis for the discovery of novel antimicrobials against XD-RAB infection.Methods Ten antipyretic-antidotal TCM were extracted with water,50％ethanol and 95％ethanol respectively,and TCM extracts with different concentrations were prepared,which were co-incubated with the model of XDR-AB-infected Caenorhabditis elegans previously optimized by the research group.The in vivo activity of antipyretic-antidotal TCM against XDR-AB infection was judged through the survival rate of Caenorhabditis elegans.Results With the increase of concentration of turmeric and cortex pseudolaricis extracts,the survival rate of XDR-AB-infec-ted nematodes continued to improve.The water extract,50％ethanol extract,and 95％ethanol extract of turmeric at a concentration of 1 000 μg/mL could increase the survival rates of XDR-AB-infected Caenorhabditis elegans to 54.2％(compared to the negative control group,P＜0.001),18.8％,and 13.3％,respectively.The water ex-tract,50％ethanol extract,and 95％ethanol extract of cortex pseudolaricis at a concentration of 1 000 μg/mL could increase the survival rates of XDR-AB-infected Caenorhabditis elegans to 47.4％(compared to the negative control group,P＜0.001),23.8％,and 15.8％,respectively.Conclusion The water extracts of turmeric and cortex pseudolaricis have good activity against XDR-AB infection,and their main chemical components can be tested for in vitro antimicrobial efficacy to discover novel antimicrobial agents against XDR-AB infection.

antipyretic-antidotal TCMextensively drug-resistant Acinetobacter baumanniiCaenorhabditis ele-gansinfection modeltraditional Chinese medicine

杨琰、冯建文、季波、袁进、王妍、李健、蔡学究、姜志辉

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南方医科大学药学院,广东广州 510515

中国人民解放军南部战区总医院临床药学科,广东广州 510010

中国人民解放军南部战区总医院药剂科,广东广州 510010

中国人民解放军南部战区总医院骨科,广东广州 510010

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清热解毒中药 泛耐药鲍曼不动杆菌 秀丽隐杆线虫 感染模型 中药

广东省自然科学基金-青年提升项目中国人民解放军南部战区总医院院内科技计划广州市科技计划-市校(院)企联合资助项目

2023A15150301532022NZA0012024A03J0644

2024

10.12138/j.issn.1671-9638.20244696

中国感染控制杂志
中南大学

中国感染控制杂志

CSTPCD北大核心
影响因子：2.112
ISSN：1671-9638
年,卷(期)：2024.23(3)
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