Chemical synthesis Dencichine and structural analysis
In L(+)-Asparagine,protected by(Boc)2O,Hofmann degradation,substitution and hydrolysis reaction to get Dencichine,HPLC 99.8%.The synthesis reaction process be monitored by TLC.Dencichine is consist β-ODAP and its α isomer α-ODAP.The 2,4-dinitrofluorobenzene pre-column derivatization liquid chromatography analysis shows that β-ODAP will transform into α-ODAP and balance in the ration of 60:40(β-ODAP:α-ODAP)when heated in the presence of water.β-ODAP and α-ODAP are also existed in original plant for the same chemical mechanism.
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