HMGB1在急性肝衰竭大鼠中的表达及作用
Expression and function of high mobility group box 1 protein in rats with actue liver failure
刘娟 1许海波 2向天新 1李细女 3邬小萍1
作者信息
- 1. 南昌大学第一附属医院，南昌，330006
- 2. 九江市第一人民医院，江西九江市，332000
- 3. 新余市第一人民医院，江西新余市，338000
- 折叠
摘要
目的：探讨HMGB1在急性肝衰竭大鼠肝组织和血中的变化规律及作用。方法将48只健康雌性SD大鼠按数字表随机分为对照组、模型组，每组24只，采用腹腔内注射D-GalN（400 mg/kg）＋LPS （100μg/kg）的方法诱导急性肝衰竭，同时对照组予腹腔注射等量生理盐水。两组大鼠均在4、8、12小时时间点取血液及肝组织标本，检测血清ALT、AST水平，HE染色观察肝组织病理变化，ELISA法检测血清HMGB1浓度，RT-PCR法检测肝组织HMGB1 mRNA表达，免疫组化法检测肝组织HMGB1的表达。结果D-GalN（400 mg/kg）＋LPS（100μg/kg）的方法能成功诱导急性肝衰竭模型。对照组血清HMGB1浓度及肝组织HMGB1 mRNA表达在各时间点均较低，而模型组随时间延长而升高。对照组肝细胞核与胞浆中仅见少量HMGB1蛋白表达，模型组肝组织HMGB1蛋白表达随时间延长升高。结论急性肝衰竭时血和肝组织中HMGB1水平均随时间延长而升高，且两者变化与肝损程度正相关。
Abstract
Objective To investigate the liver tissue and blood of HMGB1 variation and function in rats with acute liver failure. Methods The 48 healthy female SD rats were randomly divided into control group (n=24), and model group (n=24). D-GalN (400 mg/kg) and LPS (100μg/kg) were uesd to manufacture of acute liver failure model by intraperitoneal injection. Model group was given D-GalN/LPS by intraperitoneal injection, simultaneously, control group was given the same amount of saline. Each groups were taken specimens at 4 h, 8 h and 12 h after ALF induction. Each group was detected for serum ALT and AST concentration, observed liver pathological changes by HE staining, evaluated serum HMGB1 concentration by ELISA assay, and determined the level of HMGB1 mRNA by RT-PCR and the protein expression level of liver tissue HMGB1 by immunohistochemistry. Results The ALF models were successfully induced by D-GalN (400 mg/kg) and LPS (100μg/kg) injection. The serum HMGB1 concentrations and the liver tissue of HMGB1 mRNA expression of control group were lower at all time points, and they increased with time in the model group. Liver nuclei and cytoplasm of the control group only saw a small amount of HMGB1 protein expression in liver tissue, the model group HMGB1 protein expression increased with time. conclusions The blood and liver tissue HMGB1 levels of rats with acute liver failure increased with time, there was a positive correlation between them and the degrees of liver damage.
关键词
急性肝衰竭/高迁移率族蛋白1/免疫组化/RT-PCRKey words
Acute liver failure/High mobility group protein 1/Immunohistochemistry/RT-PCR引用本文复制引用
出版年
2014
NETL
NSTL
维普
万方数据