Preparation of the injectable calcium-containing bone matrix material and evaluation of its bone repair
Objective To prepare an injectable calcium-containing bone matrixs(ICBM)and evaluate its bone repair,as well as its possibility of clinical application.Methods Glycerol and gelatin were used to prepare the excipient,and then mixed with the prepared CBM powder to obtain the ICBM.The pH and calcium content of bone powder,excipient and ICBM were tested;injection thrust of excipient and ICBM were measured;all data above were used to perform the preliminary characterization of the ICBM.The biocompatibility of ICBM was evaluated by in vitro cell proliferation experiment.ICBM was implanted into Kunming mice and albino guinea pigs to evaluate the in vivo safety.Further,bone induction in vivo was verified in nude mice.Finally,rabbit femoral condyle defective model was used to study the effectiveness of bone defect repair.Results The pH and calcium content of bone powder was(6.51±0.32)and(26.24±1.25)%;the pH and injection thrust of excipient was(5.56±0.28)and(1.23±0.16)N;the pH,calcium content and injection thrust of ICBM was(6.44±0.47),(5.32±0.73)%and(10.87±1.53)N.The relative cell proliferation rate of L929 cell for the ICBM group was(104.25±3.39)%,and the cytotoxicity rating of the ICBM was level 0.There was no acute systemic toxicity in Kunming mice,and no delayed hypersensitivety in albino guinea pigs.Histological observation of bone induction in vivo showed that the ICBM group exhibited significant osteogenic phenomena.The repaired area of rabbit femoral condyle defect at week 8 were(12.91±2.27)%in DBM group and(15.68±2.25)%in ICBM group,while the differences were statistically significant between the two groups.Conclusions An ICBM was prepared to make CBM injectable without affecting the osteogenic activity.It is easy and safe,while clinical application is recommended.
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NSTL
万方数据
维普
