Effect of platelet-rich fibrin on the proliferation and differentiation of rat epiphyseal plate chondrocytes
Objective To study the effect of platelet-rich fibrin(PRF)on the proliferation and differentiation of rat epiphyseal plate chondrocytes cultured in vitro.Methods Four 2-week-old SD rats were selected,and primary epiphyseal plate cells were obtained and subjected for culture and identification.10 ml whole blood of 8-week-old rats was collected to prepare PRF.PRF was not added in the medium of the control group;PRF membrane was added at the bottom of the medium of the experimental group.CCK-8,scratch experiment and sulfate glycosamine polysaccharide content glycosaminoglycan(GAG)were used to evaluate the proliferation,migration and biological function activity of cells with PRF.Results After 48h of PRF treatment in the experimental group,the CCK-8 test showed a significantly higher proliferation rate compared with the control group(P<0.05).After 24 h and 48 h of PRF treatment,the scratch experiment indicated that the wound closure of experimental group were(81.27±3.02)%,(97.60±0.89)%,which were significantly higher than the control group[(28.04±4.50)%,(45.68±2.44)%](P<0.05).After 24 h of PRF treatment in the experimental group,there was no statistical difference in GAG content compared with the control group(P>0.05).After 48 h of PRF treatment in the experimental group,the GAG content(102.45±4.11)μg/ml was significantly increased than that of the control group(56.62±4.61)μg/ml(P<0.05).Conclusions PRF can promote the proliferation and differentiation of epiphyseal plate chondrocytes and GAG production.
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