Acinetobacter calcoaceticus PHEA-2 separated from the wastewater of oil refinery has rather strong ability of phenol degradation. Under the conditions of 30℃ and incubation amount of 1%, it can degrade 300mg/L phenol entirely within 24 hours; and the optimal temperature for its growth and phenol degradation is 30℃. The degradation ability can maintain at the range from pH5 to pH10. The degrading reaction of Acinetobater calcoaceticus PHEA-2 accords with Monod model. The reaction constant Km is 275mg/L; and the greatest reaction speed Vm is 41mg phenol/(108·h). Glucose applied at 5% can stimulate the growth of PHEA-2 and enhance the degrading ability of PHEA-2 culture system.