Effect of dissolve oxygen on the microbial community of the nitrite-oxidizing bacteria in an intermittent aeration reactor
Nitrospira andNitrobacter are two dominant types of nitrite-oxidizing bacteria (NOB).To evaluate the effect of dissolved oxygen (DO) on microbial community of NOB under the intermittent-aeration mode, the population and microbial community of this two NOB types were investigated in an aeration reactor with low DO period (55d) and high DO period (113d). Results showed the population ofNitrospira was much higher than that ofNitrobacter during the low DO period (0.2~0.5mg/L), and most clones ofNitrospira was very similar to the cloneCandidatus Nitrospira defluvii. After increasing DO to a high level (1.5~2.3mg/L) and operating for 113d,Nitrobacter turned to the dominant group. Most clones ofNitrobacter were distributed to the branch containinNitrobacter winogradskyi. Furthermore, the nitrite accumulation occurred in this reactor during the shift of population betweenNitrospira andNitrobacterafter increasing DO, and it gradually disappeared when Nitrobacter became the dominant NOB group.