Effect of continuous shock load on the performance and microbial community responses of anaerobic sequencing biofilm batch reactor
A laboratory-scale anaerobic sequencing biofilm batch reactor(AnSBBR)was used for the digestion of winery wastewater,and its operational characteristics and microbial community structure under continuous shock load were investigated.The results showed the AnSBBR maintained high removal efficiency and stability during the first 13days(29～41d)of continuous shock load.With the increase of hydrogen partial pressure and VFAs contents,the stability decreased(VFA/TA=0.72),SMA decreased by 46.2％,HUR increased by 69.2％,and coenzyme F420concentration increased by 11.9％.Compared with the steady stage,only the Slime-EPS contents increased significantly(34.1％)at day 42,while at day 56,the TB-EPS and LB-EPS contents increased by 61.3％and 62.8％,respectively,and the PN/PS ratio increased by 197.8％and 126.0％.Continuous shock load induced EPS to release massive emergency protein products to resist stress,and the electroactive substance enhanced the electron transfer activity(35.5％).Illumina MiSeq has shown that the abundance of acidogenic bacteria such as Desulfovibrio,Ruminococcus,and Geobacter decreased,while the abundance of Methanobacterium increased from 32.2％to 50.9％under continuous shock load.The biofilm system responds to the impacts of continuous shock load by EPS layered secretion and enhanced the methanogenic pathway utilizing CO2 and H2.
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