摘要
构建光序批式反应器(PSBR)处理模拟生活污水,考察光合细菌投加量、pH 值、光照强度和溶解氧(DO)对反应器污染物去除性能影响,通过 16S rRNA 测序技术揭示光合细菌对活性污泥微生物群落结构、功能和氮代谢通路的影响.结果表明:10%光合细菌投加通过促进微生物种间协调功能使PSBR系统CODCr、NH4+-N和TN去除率提高,pH值、光照强度和DO分别为7、5000lux和3mg/L时,PSBR脱氮性能最好.光合细菌提高了活性污泥微生物多样性和丰富度,Proteobacteria 菌门和 norank_f__Saprospiraceae 菌属相对丰度增多.光合细菌促进部分参与硝化过程和反硝化过程的功能基因(amo、hao和nap)和酶(AMO、HAO和NAP)丰度增加,活性污泥的整体氮代谢潜力得到提升.
Abstract
The phototrophic bacteria were introduced into the Photoheterotrophic Sequencing Batch Reactor(PSBR)to treat simulated domestic wastewater.The effects of the amount of phototrophic bacteria,pH,light intensity,and dissolved oxygen(DO)on the pollutant removal performance of the reactor were investigated,and the impact of phototrophic bacteria on the microbial community structure,function,and nitrogen metabolism pathway of the active sludge microorganisms was revealed through 16S rRNA sequencing technology.The results showed that the addition of 10%phototrophic bacteria improved the removal rates of CODCr,NH4+-N,and TN in the PSBR system by promoting the coordinated functions between microorganisms.The optimum pH,light intensity,and DO for nitrogen removal performance in the PSBR were determined to be 7,5000lux,and 3mg/L,respectively.The introduction of phototrophic bacteria enhanced the microbial diversity and abundance in the active sludge,with an increase in the relative abundance of Proteobacteria phylum and norank_f__Saprospiraceae genus.The abundance of functional genes(amo,hao,and nap)and enzymes(AMO,HAO,and NAP)involved in partial nitrification and denitrification processes was increased by phototrophic bacteria,thereby enhancing the overall nitrogen metabolism potential of the active sludge.
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