黄河内蒙段开河期细菌群落结构特征研究

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中文摘要：为探索黄河内蒙段开河初期水-土介质中细菌群落组成情况及生态网络结构，于2021年4月中旬进行取样，共获得黄河水样6个和土样4个。利用16S rRNA基因高通量测序方法对水、土样品进行测定，并从菌门及菌属水平对二者的细菌群落组成进行了分析。结果显示:河漫滩土样细菌群落结构的分布较水样更为分散，且土样中细菌群落丰富度、均匀度及多样性均高于水体，二者多样性存在显著的组间差异;在菌门水平上，变形菌门、放线菌门和酸杆菌门等是构成水-土样品的优势细菌门类，其中变形菌门在河水中相对丰度均值约为 36。57%，在土样中则下降到 25。39%，而酸杆菌门主要存在土样中，相对丰度约为14。17%;在菌属水平上，hgcI_clade(6。19%～12。75%)、CL500-29_marine_group(2。97%～5。05%)和Acinetobacter(0。74%～12。24%)是水体中的优势菌属，而 norank_f__norank_o__norank_c__KD4-96(1。95%～4。91%)、norank_f__norank_o__Vicinamibacterales(2。35%～4。67%)和norank_f__Gemmatimonadaceae(2。20%～4。73%)是土样中的优势菌属;不同菌属对水-土细菌群落结构的响应并不一致，其中分别归属于酸杆菌门和芽单胞菌门的 norank_f__norank_o__Vicinamibacterales 及 norank_f__Gemmatimonadaceae 等是联系水-土细菌群落的主要菌属，而归属于放线菌门的hgcI_clade和CL500-29_marine_group等是造成水、土优势菌门存在差异的主要菌属;虽然土样较水样拥有更大的生态网络结构，且Delftia、Candidatus_Methylopumilus、Arthrobacter和Rb41 等是维持黄河内蒙段水、土介质生态网络结构的关键菌属，但相较于土样网络结构，水体网络结构中不同模块关键菌门及菌属间的生态功能较为一致。

外文标题：Characterization of bacterial community structure during the opening period of the Yellow River in Inner Mongolia

外文摘要：In order to explore the bacterial community composition and ecological network structure in the water-soil medium at the beginning of the opening of the Inner Mongolia section of the Yellow River,sampling was carried out in mid-April,2021,a total of 6 water samples and 4soil samples were obtained from the Yellow River.The samples were measured by 16S rRNA high-throughput sequencing,which analyzed the bacterial community composition of the water and soil samples at the level of phylum and genus.The results showed that∶ the distribution of soil bacterial community structure was more dispersed than that of water,the abundance,uniformity and diversity of bacterial communities in soil samples were higher than those in water.There were significant inter-group differences in diversity between the two.At the phylum level,Proteobacteria,Actinobacteria and Acidobacteriota were the dominant bacterial phyla in water and soil samples,and the mean relative abundance of Proteobacteria in river was about 36.57%,while that in soil decreased to 25.39%;Acidobacteriota was mainly present in soil with a relative abundance of about 14.17%.At the genus level,hgcI_clade(6.19%～12.75%),CL500-29_marine_group(2.97%～5.05%)and Acinetobacter(0.74%～12.24%)were the dominant genera in water,while norank_f__norank_o__norank_c__KD4-96(1.95%～4.91%),norank_f__norank_o__Vicinamibacterales(2.35%～4.67%),and norank_f__Gemmatimonadaceae(2.20%～4.73%)were the dominant genera in soil.The response of different genera to the community structure of soil and water bacteria was not consistent,among which belonged to the phylum Acidobacteriota and Gemmatimonadota were the main genera linking the soil and water bacterial community,respectively,while hgcI_clade and CL500-29_marine_group belonging to Actinobacteria were the main genera causing the difference of the dominant phylum of soil and water.Although soil had a larger ecological network structure than water,and Delftia,Candidatus_Methylopumilus,Arthrobacter and Rb41 were the key genera that maintained the ecological network structure of the soil-water medium in the Inner Yellow River,the ecological functions of different modules in the network structure of the water were more consistent comparing to soil.

外文关键词：

bacterial communitywater-soil mediumnetwork Structureriver opening periodInner Mongolia section of the Yellow River

作者：

史玉娇、李文宝、郭鑫

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作者单位：

内蒙古农业大学水资源利用与保护自治区重点实验室,内蒙古呼和浩特 010018

黄河流域内蒙段水资源与水环境综合治理协同创新中心,内蒙古呼和浩特 010018

关键词：

细菌群落水土介质网络结构开河期黄河内蒙段

基金：

内蒙古自治区科技重大专项内蒙古自治区自然科学基金资助项目内蒙古自治区高等学校青年科技英才支持计划内蒙古自治区科技攻关项目国家自然基金资助地区项目

项目编号：

2020ZD00092021MS05043NJYT-20-A142020GG000952160021

出版年：

2024

中国环境科学

中国环境科学学会

中国环境科学

CSTPCDCHSSCD北大核心

影响因子：2.174

ISSN：1000-6923

年,卷(期)：2024.44(3)

参考文献量29