To enhance the efficiency of microbial degradation of PAEs in the environment,this study immobilized three strains,namely Glutamicibacter sp.A4,Bacillus sp.W34and Rhodococcus sp.2G,on biochar though adsorption method.The degradation efficiency of strains on six PAEs was investigated,and the metabolic pathway of PAEs degradation was preliminarily analyzed.The results showed that the complex flora achieved an 80.04%degradation rate of 120mg/L ∑6PAEs after 7days.Under optimal conditions(2days immobilization at 30℃,and a bacteria-material ratio of 1∶30(V/M)),the prepared immobilized bacterial agent achieved a 96.60%degradation rate of ∑6PAEs,which was16.56%higher than that of free bacteria.During the degradation of DBP and BBP by complex bacteria,several metabolites,including monomethyl phthalate(MMP)and monoethyl phthalate(MEP),were produced.This suggested that ester hydrolysis was an important biodegradation process for PAEs.The findings of this study provided a theoretical basis for the application of immobilized bacterial agents in the remediation of environmental organic pollution.
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