Influence of hydrous oxides on the horizontal transfer of antibiotic resistance genes
This study explored the effects of hydrous oxides of aluminum and hydrous oxides of manganese on the horizontal transfer of ampicillin resistance genes carried by pUC19 plasmid to recipient E.coli cells,respectively.In the concentration range of 2.5～200mg/L,hydrous oxides significantly inhibited the horizontal transfer of antibiotic resistance genes(ARGs).As compared to the control group,inhibition rates of hydrous oxides of aluminum and hydrous oxides of manganese on transformation frequencies were up to 79.44%and 57.64%,respectively.The Al(Ⅲ)released from hydrous oxides of aluminum are one of the reasons for the influenced ARG transformation,and the release of cation from hydrous oxides of manganese was negligible.In addition,hydrous oxides of aluminum can form large agglomerates with pUC19 plasmids by binding to their phosphate groups and bases of adenine,thymine,and guanine,thus hindering the entrance of ARGs to the cells.For hydrous oxides of manganese,they significantly decreased the membrane permeability of the recipient cells and also formed large-sized complexes with plasmids,which might be the mechanisms accounting for the inhibited ARG transfer.
soilhydrous oxidesantibiotic resistance geneshorizontal gene transfer
万方数据
维普
