Combined toxicity of nanoplastics and arsenic to zebrafish(Danio rerio)
In this study,the zebrafish was exposed to 5mg/L polystyrene nanoplastics(PS-NPs),1,10 and 100μg/L of arsenic(As)as single and combined pollutants for 7 days by using semi-static waterborne exposure experiment.The research assessed As accumulation in zebrafish liver,intestine,gill and muscle tissues under the influence of PS-NPs,PS-NPs'fluorescence intensity,and stress responses in liver and gill tissues.Results revealed PS-NPs'enrichment in liver and gill tissues,enhancing As accumulation across various zebrafish tissues.Under the combined pollution of 5mg/L PS-NPs and 100 μg/L As,As content in liver,gill,intestine and muscle tissues increased by 35.18％,147.33％,163.12％and 66.96％,respectively,compared to single As exposure.Co-exposure to PS-NPs and As increased GST and GPx activities,reducing oxidative stress in liver tissues.However,in gill tissues,GSH content and GST activity decreased,while MDA content increased,indicating increased oxidative stress.Exposure to a high concentration(100μg/L)of As combined with PS-NPs significantly decreased AChE activity in gills.Mixed exposure reduced the expressions of gpx,Mn-sod,Cu/Zn-sod and ache genes in liver and gill tissues compared to single As exposure.PS-NPs were found to inhibit the expression of genes associated with As-induced oxidative stress and neurotoxicity in zebrafish.
万方数据
维普
