Efficacy and mechanism of inactivation of fungal spores by ultraviolet combined with performic acid process
The inactivation efficiency and mechanism of ultraviolet/performic acid(UV/PFA)combined process against two typical pathogenic fungi(Aspergillus Niger and Aspergillus flavus)were investigated in this study.It was found that UV/PFA could inactivate 1.21-log of Aspergillus Niger spores and 2.08-log of Aspergillus fllavus spores at 8min,and the inactivation efficiency of combined inactivation was higher than the sum of the inactivation efficiency of PFA and UV alone,showing a synergistic effect.The mechanism of combined inactivation was mainly that UV activated PFA to generate strong oxidizing hydroxyl radicals(·OH)to cause damage to cell membranes,increased the level of intracellular reactive oxygen species to increase cellular stress,and also facilitated the penetration of PFA into the cell interior,thus enhancing the inactivation efficiency.UV played a major role in the combined inactivation process,followed by·OH,which contributed 87％and 11％to Aspergillus Niger inactivation and 71％and 27％to Aspergillus flavus inactivation,respectively.In addition,after UV/PFA inactivation,the regeneration time of fungi was delayed and the maximum potential of regeneration growth was inhibited.
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