Phosphorus accumulating organisms(PAOs)are the main functional microorganisms that play the role of phosphorus removal in the enhanced biological phosphorus removal(EBPR)process.Their metabolic behavior determines the stability of the phosphorus removal system and the final phosphorus removal efficiency.In order to adapt to the external environment change,the metabolic mode of PAOs will shift from polyphosphate accumulating metabolism to glycogen accumulating metabolism.On the basis of stoichiometric parameters,the mechanism of the metabolic shift of PAOs were described and the environmental factors that may cause the metabolic shift,such as influent phosphorus concentration,metal ions,pH value,temperature,etc.were summarized in this paper.Also,the reasons for the metabolic shift in different PAOs species were classified and described.This review provides a reference for the application of PAOs in EBPR processes.