Nitrogen removal characteristics and bioenhancement effects of aerobic denitrifying bacteria Burkholderia sp.ZH8
Aiming at the problems of complicated process and low denitrification efficiency of traditional biological denitrification treatment of nitrogen-containing wastewater,an aerobic denitrification strain ZH8 was screened from a stable biological filter in the laboratory,and this strain had strong heterotrophic nitrification and aerobic denitrification ability.Strain ZH8was identified as Burkholderia sp.by morphological,physiological and biochemical characteristics and 16S rDNA sequence analysis.The single factor experiments showed that the optimal culture conditions of aerobic denitrification strain ZH8were as follows:carbon source was sodium succinate,C/N was 15,temperature was 30℃,rotational speed was 160r/min and pH was 7～8.In addition,strain ZH8 could not only grow with NH4+-N,NO3--N or NO2--N as a single nitrogen source,but also achieve simultaneous nitrification and denitrification under mixed nitrogen source conditions.According to the whole genome analysis,heterotrophic nitrification of strain ZH8 was ammonium assimilation through glutamate dehydrogenase pathway,and aerobic denitrification pathway was NO3--N→NO2--N→NO→N2O→N2.Carbon metabolism pathway included tricarboxylic acid cycle,glyoxylic acid cycle,glycolysis and pentose phosphate.In the biofilm reactor cultured with pure bacterium ZH8,the reaction could be completed after 8 to 15 days,and the system could maintain efficient and stable nitrogen removal performance,and the removal rates of NH4+-N,NO3--N and TN reached more than 90％.The strain provides a new strain resource for wastewater bio-treatment,which can effectively treat nitrogen-containing wastewater and has practical application value.
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