Treatment of nitrogen-containing wastewater by dissimilatory nitrate reduction to ammonium coupled with anaerobic ammonium oxidation
To investigate the feasibility and long-term performance of simultaneous removal of ammonium(NH4)and nitrate(NO3-)in wastewater by anaerobic ammonia oxidation(anammox)bacteria,batch,inhibitor,isotopic and long-term reactor experiments were conducted based on the intracellular carbon-dependent dissimilatory nitrate reduction to ammonium(DNRA)of anammox bacteria.In 12h batch experiments,NH4+and NO3-were simultaneously converted to N2 with anammox biomass concentration of 4500mg/L,and the total nitrogen removal efficiency was 93.49％.In the experiment of acetylene inhibition(inhibiting the process of generating hydrazine from NH4+and nitric oxide in the anammox reaction),17.01mg N/L of NH4+was detected,and the contribution rate of anammox bacteria to NO3-removal was 85.88％.After the addition of penicillin G(an inhibitor for most heterotrophic denitrifying bacteria),NO3-could still be removed.Both 29N2 and 30N2 was always detected from the very beginning in the 15N isotope tracing experiment,and the former was 1.4 folds higher than the latter.In the long-term experiment,the average NH4+and NO3-concentrations of effluent were 1.18 and 2.86mg N/L respectively,reaching an average total nitrogen removal efficiency of 92.26％.These results suggested that NH4+and NO3-could be simultaneously removed by endogenous DNRA coupled with normal anammox metabolism,further promoting the application of anammox-based processes for nitrogen-contained wastewater removal.
anammoxdissimilatory nitrate reduction to ammoniumammonianitrate
万方数据
维普
