Optimization and product characteristics of cyanobacteria-based plant growth stimulants by acid and thermal hydrolysis
This study utilized cyanobacteria as a raw material,subjecting it to acid-thermal hydrolysis.The optimal hydrolysis conditions for cyanobacteria were analyzed,and the safety of applying cyanobacteria-based plant stimulants to soil was evaluated using Pij、Igeo、RI and human exposure health risk assessment.Results demonstrated that using 2%oxalic acid for hydrolysis,exploiting the chelation effect of oxalic acid on heavy metals,and further adjusting the pH to 0.8 with sulfuric acid while maintaining a 24-hour hydrolysis constituted the optimal acid-thermal hydrolysis conditions to minimize environmental risks associated with cyanobacteria-based plant stimulants.The Pij、Igeo、RI of cyanobacteria-based plant stimulants prepared under various conditions indicated negligible risks,absence of pollution,and low-risk levels,respectively.Moreover,the calculated human carcinogenic and non-carcinogenic risks associated with plant stimulants were found to be below the safety thresholds of 10-6 and 1.In addition,using a semi-continuous acid-thermal hydrolysis system to enhance the concentration of beneficial substances in cyanobacteria-based plant growth stimulants,it was observed that after 3~4cycles of hydrolysis enrichment,the levels of nutrients(N,P,and K)in the plant growth stimulants stabilized at 3942,290.45,and 421.7mg/kg,respectively.Furthermore,2-(5H)-furanone and pyrrole compounds,classified as bio-stimulants,continued to accumulate in the plant growth stimulants,reaching levels of 29.5646 and 11.261mg/kg,respectively.These substances can effectively promote plant growth and inhibit pathogens.Moreover,alkaloids and phenolic compounds,considered as allelopathic substances,were degraded during hydrolysis,and subsequently alleviating the inhibitory effects on plant growth.