摘要
采用16S rRNA高通量测序技术分析叶际细菌群落多样性与丰富度,使用PICRUSt2 软件基于基因测序结果对叶际细菌的氮代谢等功能基因进行预测,并测定叶际细菌的反硝化速率.结果显示,石楠植物叶际细菌以变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)为优势菌门,以无色杆菌属(Achromobacter)、伯克霍尔德氏菌属(Burkholderia-Caballeronia-Paraburkholderia)、薄层菌属(Hymenobacter)为优势菌属,且同一地区的不同点位优势菌相同;同时发现石楠叶际细菌中存在无色杆菌属(Achromobacter)、马赛菌属(Massilia)和鞘氨醇单胞菌属(Sphingomonas)等具有反硝化功能的菌属;PICRUSt2功能基因预测叶际细菌6个一级功能层中以代谢功能为主,30个二级功能层中以膜转运功能为主;氮代谢功能基因中反硝化相关基因丰度最高,固氮相关基因丰度最低;测得叶际细菌反硝化速率为(22.2±1.7)～(33.2±4.7)µg/(g·h).
Abstract
In this study,16S rRNA high-throughput sequencing technology was used to analyze the diversity and richness of the phyllosphere bacterial community,and PICRUSt2 software was used to predict the nitrogen metabolism and other functional genes of the phyllosphere bacteria based on the gene sequencing results,and the denitrification rate of the phyllosphere bacteria was measureed.The results showed that Proteobacteria,Actinobacteria,and Bacteroidetes were the dominant phyla in the phyllosphere bacteria of Photinia plants,and Achromobacter,Burkholderia-Caballeronia-Paraburkholderia,and Hymenobacter were the dominant genera,and the dominant bacteria were the same at different locations in the same region.In addition,it was also found that Achromobacter,Massilia,and Sphingomonas,which have denitrification functionsthere,distributed in the phyllosphere of Photinia.The PICRUSt2prediction showed that the metabolic function pathway is dominant of the 6primary functional layers of phyllosphere bacteria,and the membrane transport function is dominant in the 30 secondary functional layers.Meanwhile,the abundance of denitrification related genes is the highest among nitrogen metabolism functional genes,and the abundance of nitrogen fixation related genes is the lowest.The measured denitrification rate of phyllosphere bacteria is(22.2±1.7)-(33.2±4.7)µg/(g·h).
维普
万方数据