The neurotoxic effects and mechanisms of titanium dioxide nanoparticles
The toxicological effects and mechanisms of different crystal forms(anatase,rutile,mixed)and particle sizes(25,50,100nm)of titanium dioxide nanoparticles(TiO2-NPs)on dopaminergic cells were thoroughly investigated using SH-SY5Y cells and PC12 cells as experimental models.The results showed that rutile TiO2-NPs exhibited pronounced toxic effects,followed by mixed and anatase TiO2-NPs.Furthermore,smaller particle sizes were associated with greater toxicity to nerve cells,particularly at 25nm.The neurotoxicity was related to the induction of reactive oxygen species(ROS)generation and apoptosis.The Western Blotting analysis revealed that TiO2-NPs upregulated the levels of Bax and Cytochrome C,downregulated the expression of Bcl-2,and activated Caspase-3,indicating that TiO2-NPs induced apoptosis of dopaminergic cells by affecting the expression of proteins related to mitochondrial apoptosis pathway.
万方数据
维普
