首页|页岩气返排水中的反硝化厌氧甲烷氧化微生物——群落结构与关键影响因子

页岩气返排水中的反硝化厌氧甲烷氧化微生物——群落结构与关键影响因子

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首次探究了高温高压高盐的页岩气返排水中DAMO(denitrifying anaerobic methane oxidation)微生物存在的证据及其与关键环境因子之间的关联性.结果表明,在所研究的 7 个页岩气井场返排水中均检测到了DAMO古菌与细菌的存在,且古菌的丰度远高于细菌,其 16S rRNA基因拷贝数分别为 4.32×104～5.83×105copies/L和 1.16×103～6.00×103copies/L.DAMO古菌与细菌的功能基因mcrA与pomA同样在所有的样品中检出.页岩气返排水中共发现了 4个DAMO细菌代表性OTU与 1个DAMO古菌代表性OTU,虽然返排水中盐度很高,但检出的DAMO细菌与古菌与已报道的淡水环境中的 DAMO 微生物亲缘性更近.RDA 相关性分析表明,返排水常规理化因子对 DAMO 微生物的影响并非体现在微生物的丰度上,而是体现在微生物的功能上,其中pmoA基因丰度与TOC含量呈正显著相关,而mcrA基因丰度则与NO3-、NH4+、PO43-和Cl-浓度呈正相关,Cl-影响最大.研究结果拓展了关于 DAMO 微生物环境分布的认识,可为缓解返排水中逸散甲烷所导致的温室效应的控制提供理论基础,也有助于加深对深部页岩微生物过程的认知.
Denitrifying anaerobic methane oxidation microorganisms in shale gas flowback water ― Community structure and key influencing factors
In this study,we investigated the presence of denitrifying anaerobic methane oxidizing microorganisms in high temperature,high pressure and high salt shale gas flowback water for the first time,along with their correlation with key environmental factors.The results indicated that DAMO archaea and bacteria were detected in all the seven shale gas flowback water,and the archaea exhibiting significantly higher abundance compared to bacteria with 16S rRNA gene copy ranging from 4.32×104∼5.83×105copies/L and 1.16×103～6.00×103 copies/L,respectively.Additionally,the functional genes mcrA and pomA of DAMO archaea and bacteria were also detected in all the samples.Four representative OTUs of DAMO bacteria and one representative OTU of DAMO archaea were identified in the shale gas flowback water.Despite the high salinity,the DAMO bacteria and archaea detected in flowback water shared a closer genetic relationship to those reported in freshwater environments.RDA correlation analysis revealed that the influence of physicochemical factors in flowback water on DAMO microorganisms was more evident in microbial function rather than microbial abundance.The abundance of pmoA gene was positively and significantly correlated with TOC content,while the abundance of mcrA gene was positively correlated with the concentrations of NO3-,NH4-,PO43-,and Cl-,with Cl-having the greatest impact.These results of the study expanded the understanding of the environmental distribution of DAMO microorganisms,providing a theoretical foundation for controlling greenhouse effect caused by methane emissions in flowback water,and contributing to a deeper comprehension of microbial processes in deep shale formations.

denitrifying anaerobic methane oxidationshale gas flowback waterhigh salinityenvironmental factorscommunity structure

李欣悦、卢培利、刘俊、李仕康、侯争鸣、丁阿强

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重庆大学煤矿灾害动力学与控制国家重点实验室,重庆 400044

重庆大学环境与生态学院,重庆 400045

重庆大学三峡库区生态环境教育部重点实验室,重庆 400045

反硝化厌氧甲烷氧化 页岩气返排水 高盐 环境因子 群落结构

国家自然科学基金资助项目国家自然科学基金资助项目国家重点研发计划项目煤矿灾害动力学与控制国家重点实验室开放项目

52070025522000862019YFC18055032011DA105287-MS202213

2024

中国环境科学
中国环境科学学会

中国环境科学

CSTPCDCHSSCD北大核心
影响因子：2.174
ISSN：1000-6923
年,卷(期)：2024.44(9)