Effects of eggshell microcarriers on the rapid cultivation of nitrifying granular sludge
The influence of adding eggshell microcarriers on the rapid cultivation of nitrifying granular sludge was investigated in a continuous flow gas-lift internal circulation reactor.The results showed that the reactor(R2)with the addition of 0.5g/L eggshell microcarriers(75μm)achieved sludge granulation within one month,which was 1~2weeks earlier compared to the control(R1).Furthermore,the resulting granular sludge in R2 exhibited higher mixed liquor volatile suspended solids(MLVSS)(R1=1.7g/L,R2=2.2g/L),Tightly Bound-EPS(TB-EPS)content(R1=97.65mg/gVSS,R2=136.29mg/gVSS),TB-PN/PS ratio(R1=2.95,R2=4.73),and better settling performance(with respective SVI3and SVI30 values of 41.67,37.5mL/g for R1,and 30.36,28.57mL/g for R2).Compared to R1,the granular sludge in R2had higher activity,with specific oxygen uptake rate(SOUR)and specific ammonia oxidation rate(SAOR)values of 754mgO2/(gVSS·h)and 90.74mg/(gVSS·h),respectively.High-throughput sequencing results indicated that the granular sludge supplemented with eggshell microcarriers had a higher abundance of nitrifying bacteria(Nitrosomonas)and EPS-producing bacteria(such as Comamonadaceae_unclassified).