The impact of enzyme enhancement on municipal sludge biological drying system
The effects of enzyme intensification were explored by adding composite enzymes(protease and amylase)to the municipal sludge bio drying system.The addition of these enzymes was found to increase the content of humus precursors and quinone functional groups,enhance the system's electron transfer ability,and promote the efficient degradation of protein and total sugar in the reactor.The rate of reaching high temperature in the bio drying system was increased by 3.75times,with the maximum temperature rising from 59.2℃ to 72.8℃,and the duration of high temperature(>50℃)increasing by 5.56times.Microbial analysis results indicated that the addition of enzymes led to an increase in the abundance of aerobic thermophilic microorganisms such as Firmicutes(Geobacillus,Ureibacillus,Bacillus),Caldibacillus(Thermobacillus).The addition of complex enzymes also enhanced the abundance of Gram-positive bacteria in the biological drying system at high temperatures,strengthened the ATP-binding cassette transporter pathway,accelerated the membrane transport process of small molecular organic matter(amino acids,peptides,carbohydrates,etc.),and improved the supply of microbial metabolizable substances,thereby providing a more sufficient substrate guarantee for the improvement of microbial efficiency in the municipal sludge biological drying system.
municipal sludgeenzymebiological dryingelectron transfer capacitycommunity structuremetabolic pathway