Isolation of a high-efficient diesel degrading bacteria and its degradation of alkane components of diesel
A diesel degrading bacterial strain 13-3isolated from oil-contaminated soil in an oilfield mining area in Northeast China was identified as Acinetobacter sp.by morphological observation and 16S rRNA.The strain could degrade 88.50％diesel of 1g/L cultured at 20℃ at 180r/min for 7days,which showed excellent diesel degrading ability compared with other strains,and the degradation rate of diesel fuel could reach 71.7％after being inoculated into 10g/kg diesel contaminated soil and remediation at 20℃ and soil moisture content of 30％for 21 days,which indicated it had great potential in practical diesel-contaminated soil remediation applications.According to the analysis of GC-MS spectrum and the degrading experiments of different chain length alkane substrates,strain 13-3had a wide alkane utilization spectrum(C14～C22),and the degradation rate of C17～C20 could reach more than 90％.The results of environmental factors showed that strain 13-3could grow in the range of pH=6～9,temperature 10～30℃,salinity(NaCl)0.5％～3％,and still had a high concentration of bacterial solution at the initial diesel concentration of 10g/L,which indicated that strain 13-3 had good environmental adaptability and was expected to exert its degradation performance under complex environmental conditions.Through whole-genome sequencing analysis,three key genes(alkB,almA,ladA)were found in the genome of strain 13-3,and the possible degradation pathway of strain 13-3 was speculated to be the terminal oxidation pathway.
strain screening and identificationAcinetobacterdiesel degradationalkane degradation
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维普
