This study employed an anaerobic slurry incubation test to evaluate whether the acetylene inhibition method may have led to an underestimation of the denitrification potential in both greenhouse vegetable and grain field soils,using a robotized sampling and analysing system.Soils from greenhouse vegetable fields and adjacent grain fields in the Shouguang region were sampled.The treatments with and without the addition of 10％acetylene were set up respectively.The results showed that the accumulated N2O concentration in the 10％acetylene treatment was significantly higher than those without acetylene after 3hours of anaerobic slurry incubation,while N2 accumulation showed the opposite trend.Moreover,in the presence of acetylene,7.92％and 34.2％of N2O were reduced to N2 in greenhouse vegetable and grain field soils,respectively,within 63 hours of anaerobic incubation.The actual denitrification potential significantly increased with the addition of 10％acetylene in the greenhouse vegetable and grain field soils,but the respiration of the soils was not significantly affected.Specifically,the acetylene inhibition method overestimated the actual denitrification potential in greenhouse vegetable soil by 22.0％,and underestimated it in grain field soil by 11.4％,as determined by the bias calculated from the acetylene inhibition method(AIT-bias).Overall,the acetylene inhibition method was found to be insufficient in completely suppressing N2O reduction to N2 in greenhouse vegetable and grain field soils.The uncertainty of acetylene inhibition method on the actual denitrification potential of soil is profoundly influenced by the cropping system,which may be related to variations in soil nutrients,types and other factors.Further research is necessary to assess the reliability of this method across different soil conditions.
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