Effect and mechanism of sulfamethoxazole/trimethoprim on nitrogen removal by solid-phase denitrification
It is unclear whether the antibiotics(i.e.sulfamethoxazole(SMX)and trimethoprim(TMP))in the tail water of wastewater treatment plants would affect the nitrogen removal performance of plant carbon source supported solid-phase denitrification(SPD)technology and its mechanism.Three continuous-flow corncob-SPD(CC-SPD)reactors were established,the nitrogen removal performance of the reactors was compared,and the influence mechanism was analyzed by the metagenomic method.The results showed that:(1)The average effluent NO3--N concentrations of the three reactors were(4.09±0.38)mg/L(Rs)(5.01±0.44)mg/L(RT)and(3.53±0.35)mg/L(Rc),respectively.50μg/L SMX/TMP had no significant effect on the nitrogen removal performance of the CC-SPD reactor;(2)SMX/TMP could significantly change the microbial community structure,but the dominant bacteria in the reactor were Proteobacteria,Bacteroidetes,and Actinobacteria.Metabolism was the main function of the microbial community,and microorganisms improved the relative abundances of functions about environmental information processing and cellular processes to adapt to SMX/TMP.50μg/L SMX/TMP did not significantly affect the relative abundances of enzymes related to electron donor generation and denitrification genes.