Correlation of nitrogen metabolism and heavy metal resistance genes in anammox sludges
To elucidate the interaction between nitrogen metabolism and heavy metal resistance in different sludge aggregates,we investigated gene abundance and correlations of nitrogen metabolism genes and heavy metal resistance genes.The gene abundance analysis revealed that the total abundance of nitrogen metabolism genes and heavy metal resistance genes in granules was higher than in flocs(by 24.5%and 5.5%,respectively)and biofilm(by 57.9%and 16.7%,respectively),indicating that granules exhibit the highest potential for nitrogen metabolism and heavy metal resistance.Further,the network analysis showed that the average degree of the granule network was 63.0%and 54.4%higher than in floc network and biofilm network,respectively,highlighting the strongest correlation between nitrogen metabolism genes and heavy metal resistance genes in granules.Therefore,the granule is the optimal sludge aggregate for anammox-based process in treating ammonia-rich wastewater containing heavy metals.
heavy metal resistancenitrogen metabolismanammoxsludge aggregates