Abstract
Paper-based biosensors are widely employed in point-of-care testing(POCT)due to their convenience,portability,low cost,and ease of use.This study reports an integrated distance-based paper biosensor fabricated with a mesoporous membrane coated with stimuli-responsive polymer.The detection of α-amylase(AMY)using amylopectin-coated mesoporous membrane is demonstrated as an example.After introducing the AMY solution,it is observed that the aqueous solution flows along the paper strip due to AMY-catalyzed hydrolysis of amylopectin.The flow distance is proportional to the concentration of AMY with a detection limit as low as 4 mU/mL.In addition,the detection of AMY is demonstrated in human serum.Furthermore,the inhibitory effect of acarbose on AMY is evaluated.This reagent-free and dispos-able biosensor allows single-step rapid detection of the analyte.This approach is very promising for the development of user-friendly,equipment-free,and cost-effective biosensors with remarkable sensitivity and excellent selectivity for disease diagnosis and hypoglycemic drug screening.
基金项目
国家重点研发计划(2021YFB3201200)
国家重点研发计划(2021YFB3201202)
山东省"泰山学者"岗位项目(tsqn201812088)
山东省自然科学基金(ZR2022YQ12)
Shandong Scientific and Technical Small and Mediumsized Enterprises Innovation Capacity Improvement Project(2022TSGC2533)
Science,Education and Industry Integration Innovation Pilot Project from Qilu University of Technology(Shandong Academy of S(2022JBZ02-04)
NETL
NSTL
万方数据