Adolescent alcohol exposure changes RNA modifications in adult brain by mass spectrometry-based comprehensive profiling analysis

Ying-Ying Chen ¹Zhu Gui ²Di Hu ³Meng-Yuan Chen ⁴Jin-Gang He ²Si-Yu Yu ⁵Yu-Qi Feng ⁴Jie Wang ⁶Bi-Feng Yuan¹

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作者信息

1. College of Chemistry and Molecular Sciences,Research Center of Public Health,Renmin Hospital of Wuhan University,Wuhan University,Wuhan 430072,China;Department of Radiation and Medical Oncology,Zhongnan Hospital of Wuhan University,School of Public Health,Wuhan University,Wuhan 430071,China;Cancer Precision Diagnosis and Treatment and Translational Medicine Hubei Engineering Research Center,Zhongnan Hospital of Wuhan University,Wuhan Research Center for Infectious Diseases and Cancer,Chinese Academy of Medi
2. Key Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,National Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics,Wuhan 430071,China
3. Phase Ⅰ Clinical Trial Laboratory,Zhongnan Hospital of Wuhan University,Wuhan 430071,China
4. College of Chemistry and Molecular Sciences,Research Center of Public Health,Renmin Hospital of Wuhan University,Wuhan University,Wuhan 430072,China
5. Department of Radiation and Medical Oncology,Zhongnan Hospital of Wuhan University,School of Public Health,Wuhan University,Wuhan 430071,China
6. Key Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,National Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics,Wuhan 430071,China;Institute of Neuroscience and Brain Diseases,Xiangyang Central Hospital,Affiliated Hospital of Hubei University of Arts and S
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Abstract

Alcohol consumption is one of the leading causes of death worldwide.Adolescence is a critical period of structural and functional maturation of the brain.Adolescent alcohol use can alter epigenetic modifica-tions.However,little is known on the long-term effects of alcohol consumption during adolescence on RNA epigenetic modifications in brain.Herein,we systematically explored the long-term effects of alco-hol exposure during adolescence on small RNA modifications in adult rat brain tissues by comprehensive liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS)analysis.We totally detected 26 modifications in small RNA of brain tissues.Notably,we observed most of these mod-ifications were decreased in brain tissues.These results suggest that alcohol exposure during adolescence may impose a long-lasting impact on RNA modifications in brain tissues.This is the first report that alco-hol use during adolescence can alter RNA modifications in adult brain.Collectively,this study suggests a long-term adverse effects of alcohol consumption on brain from RNA epigenetics angle by comprehensive mass spectrometry analysis.

Key words

Mass spectrometry/RNA modification/Adolescence/Alcohol exposure/Brain

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基金项目

国家重点研发计划(2022YFA0806601)

国家重点研发计划(2022YFC3400700)

国家自然科学基金(22277093)

国家自然科学基金(22074110)

国家自然科学基金(21721005)

Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCGW-2022-008)

Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(ZNJC202208)

出版年

2024

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCDCSCD

影响因子：0.771

ISSN：1001-8417

参考文献量3

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