首页|Triplex-structure based DNA circuits with ultra-low leakage and high signal-to-noise ratio

Triplex-structure based DNA circuits with ultra-low leakage and high signal-to-noise ratio

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DNA circuits are powerful tools in various applications such as logical computation,molecular diagnosis and synthetic biology.Leakage is a major problem in constructing complex DNA circuits.It directly af-fects the output signal and harms the circuit's performance significantly.In the traditional DNA circuits,the gate complex is a duplex structure.There are insufficient energy barriers to prevent spontaneous de-tachment of strands,resulting in a leak prone.Herein,we have developed triplex-structure based DNA circuit with ultra-low leakage and high signal-to-noise ratio(SNR).The triplex structure improves the stability in the absence of input.At the same time,the driving force of the strand displacement cascades reduces the influence of the triplex structure on the desired reaction.The SNR of the DNA circuit was increased to 695,while the desired reaction rate remained 90％of the conventional translator circuit.The triplex-structure mediated leakage prevention strategy was further tested at different temperatures and in DNA translator and seesaw circuits.We also constructed modular basic logic gates with a high effi-ciency and low leakage.On this basis,we further constructed triplex-structure based tertiary DNA logic circuits,and the SNR reached 295,which,to the best of our knowledge,was among the highest of the field.We believe that our scheme provides a novel,valid,and general tool for reducing leakages,and we anticipate that it will be widely adopted in DNA nanotechnology.

LeakageDNA strand displacement cascadesTriplexDNA circuitsMolecular programming

Huan Liu、Zhihao Ming、Yuanpeng Zhang、Qidong Xia、Hao Hu、Ruijie Liu、Yuheng Liao、Yizhou Liu、Xiao Liu、Xiaoping Zhang、Longjie Li、Shaogang Wang、Xianjin Xiao

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Institute of Reproductive Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

Department and Institute of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

Department and Institute of Urology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430030,China

Department of Laboratory Medicine,Tongji hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

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国家自然科学基金国家重点研发计划Open Research Fund of State Key Laboratory of Bioelectronics,southeast UniversityOpen Foundation of NHC Key Laboratory of Birth Defect for Research and Prevention(Hunan Provincial Maternal and Child HealthOpen Foundation of Translational Medicine National Science and Technology Infrastructure(Shanghai)Open Fund from Key Laboratory of Cellular Physiology(Shanxi Medical University)教育部项目

818717322021YFC2701402Sklb2021-k06KF2020007TMSK-2021-141CPOF202103

2024

10.1016/j.cclet.2023.108555

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(1)
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