Abstract
Although it has been developed for many years,nucleic acid aptamer screening technology still fails to be widely used,a considerable part of it is due to the variability of tumor cell morphology,which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased body.To address this,primary cells that can be stably passaged were iso-lated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this study.Next,an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells,and the obtained nucleic acid aptamers were affinity verified at the cellular level.Finally,to an-swer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers,we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model,and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.
基金项目
国家重点研发计划(2017YFA0205301)
国家重点研发计划(2018YFC1602905)
国家自然科学基金(62071119)
国家自然科学基金(62075098)
国家自然科学基金(81902153)
国家自然科学基金(61527806)
Open Funding of State Key Laboratory of Oral Diseases(SKLOD2022OF05)
维普
万方数据