Evaluation of erastin synergized cisplatin anti-nasopharyngeal carcinoma effect with a glutathione-activated near-infrared fluorescent probe

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外文摘要：Nasopharyngeal carcinoma(NPC),a malignant tumor originating from the nasopharynx,is one of the common malignant tumors of the head and neck.There are significant geographical differences in the incidence of nasopharyngeal carcinoma,with a high incidence in China and Southeast Asian countries.Herein,we designed and synthesized a novel near-infrared fluorescent(NIRF)probe to detect glutathione(GSH)in cellular and tumor environments using semi-naphthofluorescein(SNAFL)as the fluorescent molecular backbone and 2-fluoro-4-nitrobenzenesulfonate as the recognition moiety.Upon reaction with GSH,SNAFL-GSH emitted a fluorescence signal,and its emission wavelength at 650 nm was remark-ably enhanced.The results of selectivity experiments indicated that SNAFL-GSH was able to discriminate GSH from Cys,Hcy,and H2S.Moreover,SNAFL-GSH could image both endogenous and exogenous GSH and distinguish normal and cancer cells by fluorescence signal difference.At the cellular level,cisplatin(DDP)-induced ferroptosis and inhibition of proliferation of various NPC cell lines(CNE2,CNE1,5-8F cells)by erastin combined with DDP were visualized with the help of SNAFL-GSH.In a mouse tumor xenograft model,we successfully employed SNAFL-GSH for the evaluation of the efficacy of erastin combined with DDP in the treatment of NPC.More importantly,the probe could image cancerous tissue sections from NPC patients with an imaging depth of approximately 80 μm.It was foreseen that SNAFL-GSH offered great potential for application in the diagnosis and evaluation of the therapeutic efficacy of NPC,and these results would also provide new ideas for the clinical treatment of NPC.

外文关键词：

GlutathioneNIRF probeFerroptosisAnti-tumor therapy evaluationBioimaging

作者：

Rong He、Dandan Tang、Ningge Xu、Heng Liu、Kun Dou、Xuejun Zhou、Fabiao Yu

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作者单位：

Department of Otolaryngology,Head and Neck Surgery,The First Affiliated Hospital of Hainan Medical University,Haikou 570102,China

Key Laboratory of Hainan Trauma and Disaster Rescue,Key Laboratory of Emergency and Trauma,Ministry of Education,Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices,College of Emergency and Trauma,Hainan Medical University,Haikou 571199,China

Sichuan Provincial General Hospital of Judicial Police,Chengdu 610225,China

基金：

Hainan Province Science and Technology Special FundHainan Province Science and Technology Special Fund国家自然科学基金国家自然科学基金国家自然科学基金海南省自然科学基金Natural Science Research Talent Project of Hainan Medical UniversityHainan Province Clinical Medical Center(2021)Project for Functional Materials and Molecular Imaging Science Innovation Group of Hainan Medical UniversityHuman nasopharyngeal carcinoma tissues were obtained from Department of Otolaryngology,Head and Neck SurgeryFirst Affiliated Hospital of Hainan Medical University

项目编号：

ZDYF2021SHFZ219ZDYF2022SHFZ288219610102226401322204037822RC831JBGS202101

出版年：

2024

DOI：

10.1016/j.cclet.2023.108658

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(2)

参考文献量55