首页|Highly efficient labelling of extracellular vesicles for enhanced detection on a microfluidic platform

Highly efficient labelling of extracellular vesicles for enhanced detection on a microfluidic platform

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Developing precise extracellular vesicles(EVs)labelling techniques with minimal disturbance is of great importance to the follow-up EVs detection and analysis.However,currently available methods such as using probes to conjugate phospholipids or membrane proteins have certain limitations due to EV steric hindrance,dye aggregation,etc.Here,we present a microfluidic platform to enhance EVs'labelling ef-ficiency and improve their detection.This platform provides excellent sample throughput and high-efficiency EV labelling at lower label concentrations with an optimized flowing rate.Flow cytometry analysis(FCM)and cellular uptake results show that EV labelling by utilizing this platform possesses the merits of a higher labelling efficiency with 64.1％relative improvement than conventional co-incubation method and a lower background noise.Moreover,this technique maintains EVs'size,morphology and biological activities.After the recipient cells uptake the EVs treated by the microfluidic platform,the spa-tial and temporal distribution of EVs in the cells are clearly observed.These results demonstrate that our method holds great potential in efficient labelling of EVs,which is essential to subsequent EV quantifica-tion and analysis.

Microfluidic platformExtracellular vesiclesLabellingPKH dyeFlow cytometry

Shi Hu、Rui Hao、Zitong Yu、Huitao Zhang、Hui Yang

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Bionic Sensing and Intelligence Center,Institute of Biomedical and Health Engineering,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

国家自然科学基金国家自然科学基金国家自然科学基金Guangdong ProgramGuangdong Basic and Applied Basic Research FoundationGuangdong Basic and Applied Basic Research Foundation深圳市科创委项目深圳市科创委项目

6207415562204253622053662016ZT06D6312020A15151109382020A1515110142KCXFZ202002011008124JCYJ20210324101405016

2024

10.1016/j.cclet.2023.108534

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(2)
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