首页|Ratiometric fluorescence probe for accurate detection of Concanavalin A by coupling fluorescent microsphere with boric acid functionalized carbon dots
Ratiometric fluorescence probe for accurate detection of Concanavalin A by coupling fluorescent microsphere with boric acid functionalized carbon dots
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Accurate and sensitive strategies for Concanavalin A(Con A)sensing are conducive to the better cognition of various important biological and physiological processes.Here,by designing dextran-functionalized fluorescent microspheres(DxFMs)and boric acid-modified carbon dots(BCDs)as recognition unit and built-in signal reference respectively,a ratiometric fluorescent detection platform was proposed for Con A detection with high reliability.In this protocol,the BCDs/DxFMs precipitation was formed due to the covalent interactions between cis-diol of DxFMs and boronic acid groups of BCDs,thus only fluores-cence of BCDs could be detected in the supernatant.When Con A was presented,it could bind to DxFMs through its carbohydrate recognition ability and suppress the subsequent assembly between DxFMs and BCDs,leading to the simultaneous capture of DxFMs and BCDs fluorescence in the supernatant.Since the BCDs content was superfluous,their fluorescence intensities were basically constant in all cases.Based on the unchanged BCDs fluorescence signal and target-dependent DxFMs fluorescence signal in supernatant,the ratiometric detection of Con A was realized.Under optimized conditions,this ratiometric fluores-cent platform displayed a linear detection range from 0.125 μg/mL to 12.5μg/mL with a detection limit of 0.089 μg/mL.Moreover,satisfied analytical outcomes for Con A detection in serum samples were obtained,manifesting huge application potential of this ratiometric fluorescent platform in clinical diagnosis.
Mingyue Xie、Juan Chen、Yufei Wang、Bojun Liu、Rong-Bin Song、Hong-Min Meng、Zhaohui Li
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College of Chemistry,Institute of Analytical Chemistry for Life Science,Henan Joint International Research Laboratory of Green Construction of Functional Molecules and their Bioanalytical Applications,Zhengzhou University,Zhengzhou 450001,China
Zhengzhou Key Laboratory of Forensic Science and Technology,Railway Police College,Zhengzhou 450053,China
School of Ecology and Environment,Zhengzhou University,Zhengzhou 450001,China
Key Project of Science and Technology of Henan Province国家自然科学基金国家自然科学基金
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