首页|Copper coordination-driven self-assembly and encapsulation of PCR reagents

Copper coordination-driven self-assembly and encapsulation of PCR reagents

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Polymerase chain reactions(PCR)are a very important tool for use in cloning,nucleic acid sequencing and diagnostic testing.The storage conditions of PCR reagents are limited to freezing and a lot of mixing steps are needed.In this paper,we report using metal ions to form coordination nanomaterials with the intrinsic components of the PCR reagents including dNTP,DNA primers and DNA polymerase as an integrated PCR reaction system.To complete PCR reactions,users need only to dissolve the coordination nanomaterials with a buffer and add template DNA.A few transition metal ions were screened and Cu2+was found to be the most effective metal ion for this purpose.Then the encapsulation efficiency of PCR reagents was measured,which can reach close to 100％for the primers and DNA polymerase,but only 10％for dNTP because dNTP was excess.Further study also exhibited this integrated PCR reaction system can be used for DNA detection with a similar detection limit to the normal PCR,and showed good stability of encapsulated PCR nanomaterial after storage for a week.

Metal ionsCoordinationEncapsulationPCRStorage

Chang Lu、Jinkai Zheng、Juewen Liu

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Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China

Department of Chemistry,Waterloo Institute for Nanotechnology,University of Waterloo,Waterloo,ON,N2L 3C1,Canada

加拿大自然科学与工程研究委员会项目国家自然科学基金国家自然科学基金Agricultural Science and Technology Innovation ProgramChina Scholarship Council(CSC)Scholarship to visit the University of Waterloo

3190177632072181CAAS-ASTIP-2021-IFST-SN2021-05

2024

10.1016/j.cclet.2023.108808

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(3)
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