Abstract
Monitoring α-glucosidase(α-Glu)activity is of great significance for the early diagnosis of type Ⅱ di-abetes.Here the blue fluorescent carbon dots(CDs)were integrated with two different recognizing molecules,β-cyclodextrin and phenylboronic acid,for assembling a multifunctional CDs(mCDs)nanoplat-form for sensitively analyzing α-Glu and its inhibitors.The hydrolyzed product of 4-nitrophenyl-α-D-glucopyranoside(α-Glu substrate),p-nitrophenol,could efficiently quench the fluorescence of mCDs due to its cooperative molecular recognition with #-cyclodextrin and phenylboronic acid.The mCDs could be utilized for the detection of α-Glu activity with the limit of detection of 0.030 U/L.Moreover,the presentα-Glu detection platform revealed a high selectivity,and other natural enzymes showed scarcely any ef-fect on the present mCDs system.The proposed method could be facilely used to screen α-Glu inhibitors with satisfying performance.The rational mCDs is expected to supplement more comprehensive biosens-ing platforms for highly sensitive and specific recognition of disease-relevant biomarkers with clinical importance.
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